Meeting Minutes: 27th June 2007

We welcomed our newest BiRG member "Kimberly Holmes"
The discussion today was about Working with a single DNA sequence : the slides are available in the Shared Folder
David began his presentation with the statement "Not everything that can be counted counts and not everything that counts can be counted"

Topics covered in detail included

- PCR
- Cloning
- Cleaning a DNA sequence (removing contamination)
- Restriction Map
- FIRTSMARKET website (use Netscape browser)
- Primer Design (MIT Website)
- Emboss
- Gene Prediction [ prokaryotes -> GeneMark ]
- Gene Prediction [ eukaryotes -> Genomescan ]
- BLAST
- Shotgun sequencing/contigs/assemble/homology
- read pieces
- PHRAP assembly program
- CAP3 program for joining small sequences

We have a nice discussion with Dr. Ramachandran explaining several concepts during the presentation. We also discussed about gene expression and Hidden Markov Models

Lastly, we do not meet next week - Independence Day - July 4